Equipment

  • 1D gel electrophoresis station (X-cell Surelock Minicell, Invitrogen)
  • Automated protein digestion station from 1D and 2D gel (ProPrep, Perkin-Elmer)
  • Mass Spectrometers : Orbitrap Elite (Thermo), Q Exactive HF (Thermo), Q Exactive Plus (Thermo), Tribrid Fusion Orbitrap (Thermo)
  • Database searches using Mascot 2.3, PEAKS 8.0, Andromeda (Maxquant) search engines, protein and peptide profiling done with custom software